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Tools for ribosomal RNA technology

Tool Description WebpageCitation
ARB A software environment for sequence data Opens external link in new window www.arb-home.de Ludwig, W., O. Strunk, R. Westram, L. Richter, H. Meier, Yadhukumar, A. Buchner, T. Lai, S. Steppi, G. Jobb, W. Forster, I. Brettske, S. Gerber, A. W. Ginhart, O. Gross, S. Grumann, S. Hermann, R. Jost, A. Konig, T. Liss, R. Lussmann, M. May, B. Nonhoff, B. Reichel, R. Strehlow, A. Stamatakis, N. Stuckmann, A. Vilbig, M. Lenke, T. Ludwig, A. Bode, and K. H. Schleifer. 2004. ARB: a software environment for sequence data. Nucleic Acid Res. 32: 1363-1371
SILVA Comprehensive, quality controlled and aligned ribosomal RNA sequence databases for all three domains of life Opens external link in new window www.arb-silva.de Pruesse, E., C. Quast, K. Knittel, B. Fuchs, W. Ludwig, J. Peplies, and F. O. Glöckner. 2007. SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB. Nucleic Acid Res. 35: 7188-7196
StrainInfo.net bioportal Virtual catalogue providing integrated downstream information on microorganisms deposited within a global network of biological resource centers Opens external link in new window www.straininfo.net Dawyndt P, Vancanneyt M, De Meyer H, J. Swings. Knowledge accumulation and resolution of data inconsistencies during the integration of microbial information sources. IEEE Transactions on Knowledge and Data Engineering, 17: 1111-1126.
RDP II The Ribosomal Database Project II Opens external link in new window rdp.cme.msu.edu Cole, J. R., Q. Wang, E. Cardenas, J. Fish, B. Chai, R. J. Farris, S. A. Kulam, D. M. McGarrell, T. Marsh, G. M. Garrity, and J. M. Tiedje. 2009. The Ribosomal Database Project: improved alignments and new tools for rRNA analysis. Nucleic Acid Res. 37:D141-145
Greengenes Ribosomal RNA database and data analysis tools Opens external link in new window greengenes.lbl.gov DeSantis, T.Z., Hugenholtz, P., Larsen, N., Rojas, M., Brodie, E.L., Keller, K., Huber, T., Dalevi, D., Hu, P. and Andersen, G.L. (2006) Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB, Appl Environ Microbiol, 72: 5069-5072
STRING Reference data of completely sequenced genomes, annotated with orthology and pathway information, plus protein network context Opens external link in new window www.string-db.org von Mering C, Jensen LJ, Kuhn M, Chaffron S, Doerks T, Krüger B, Snel B, Bork P. 2007. STRING 7--recent developments in the integration and prediction of protein interactions. Nucleic Acids Res. 35: D358-62
RAMI A software for the identification and the visualization of microdiverse sequence clusters, based on patristic distances

Opens external link in new window www.acgt.se/RAMI
(publicly available
after publication)

Pommier, T., Canbäck, B., Lundberg, P., Hagström, Å and Tunlid, A. RAMI, a new tool to characterize microdiverse clusters in microbial communities: application to ribosomal DNA sequences from the marine environment. In preparation
(MICRO)-CARD-FISH Microautoradiography combined with catalyzed reporter deposition fluorescence in situ hybridization with a cell permeabilization procedure particulary suited for Archaea

Opens external link in new window www.microbial-

oceanography.eu

Teira, E., T. Reinthaler, A. Pernthaler, J. Pernthaler, G.J. Herndl, 2004: Combining catalyzed reporter deposition-fluorescence in situ hybridization and microautoradiography to detect substrate utilization by Bacteria and Archaea in the deep ocean. Appl. Environ. Microbiol., 70: 4411-4414
Q-MICRO-CARD-FISH Quantification of single cell activity using microautoradiography combined with catalyzed reporter deposition fluorescence in situ hybridization and image analysis

Opens external link in new window www.microbial-

oceanography.eu

Sintes, E., G.J. Herndl, 2006: Quantifying substrate uptake of individual cells of marine bacterioplankton by catalyzed reporter deposition fluorescence in situ hybridization combined with microautoradiography. Appl. Environ. Microbiol., 72: 7022-7028
BOLD

BOLD is a resource for the DNA barcode community

Opens external link in new window www.barcodinglife.org/

docs/boldtutorial.html

Sujeevan Ratnasingham and Paul D.N. Hebert - University of Guelph
released 13. May 2008